Malate Dehydrogenase Assay Kit ยี่ห้อ Sigma

PROPERTIES

detection method

colorimetric

storage temp.

−20°C

Gene Information

human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)

mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)

rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)

DESCRIPTION

General description

Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.

Application

Malate Dehydrogenase Assay Kit has been used in LDH (lactate dehydrogenase) and malate dehydrogenase (MDH1) assays.[1] It has also been used to estimate isocitrate dehydrogenase[2] and succinate dehydrogenase activities.[3]

Suitability

Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.