Cathepsin b inhibitor screening kit ยี่ห้อ Sigma

PROPERTIES

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... CTSB(1508)

mouse ... CTSB(13030)

rat ... CTSB(64529)

DESCRIPTION

General description

Cathepsin B (CTSB; EC 3.4.22.1) is a lysosomal cysteine proteinase involved in intracellular degradation and turnover of proteins. It is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP is a causative factor in Alzheimer′s disease. CTSB has also been implicated in the invasion and metastasis of certain cancers.

The Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (λex = 400/λem = 505 nm), proportional to the enzymatic activity present.

Suitability

Suitable for the screening of inhibitors of Cathepsin B.

Principle

The Cathepsin B Inhibitor Screening Kit is a simple assay for screening potential inhibitors of CTSB. CTSB activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (λex = 400/λem = 505 nm), proportional to the enzymatic activity present.