Enteropeptidase/Enterokinase Inhibitor Screening Kit ยี่ห้อ Sigma
รหัสสินค้า : MAK205
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PROPERTIES
detection method
fluorometric
storage temp.
−20°C
DESCRIPTION
General description
Enteropeptidase (Enterokinase; EC 3.4.21.9) is an enzyme that converts trypsinogen to trypsin, the activity of which is required for the activation of chymotrypsin and procarboxypeptidases. It has an important role in food digestion and may serve as a marker of differentiated enterocytes and goblet cells.
Suitability
Suitable for the screening of inhibitors of Enteropeptidase/Enterokinase.
Principle
The Enteropeptidase/Enterokinase Inhibitor Screening Kit is a simple assay for screening potential inhibitors of enteropeptidase. Enteropeptidase activity is measured by cleaving a synthetic 7-amino-4-trifluoromethyl-coumarin (AFC)-based substrate to yield AFC, a fluorescent product (λex = 380/ λem = 500 nm), proportional to the enzymatic activity present. The substrate includes the ′DDDDK′ recognition sequence for enteropeptidase.
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