Chloramphenicol Extraction Kit, ยี่ห้อ Sigma

PROPERTIES

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Waste Prevention

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Aligned

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room temp

DESCRIPTION

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.

Application

The Chloramphenicol Extraction Kit is intended for the extraction of chloramphenicol from food and biological samples. It can be used for any biological food sample - shrimp, meat, liver, crab, fish, etc.

Click on the following link for a video demonstration of how the kit works

Features and Benefits

The Chloramphenicol Extraction kit shortcuts the extraction process by eliminating the need for centrifugation and manual pipetting. After that, the extract can be used to detect chloramphenicol by ELISA and spectrophotometric analysis.

Suitability

This kit is suitable for the extraction of chloramphenicol from a variety of samples such as biological tissue and food.

Principle

Chloramphenicol, or chlornitromycin, is a broad-spectrum antibiotic that is used to treat a wide variety of bacterial infections. However, due to potential serious side effects its use has been banned in many countries. Minimum acceptable limits have been set for chloramphenicol residues in food samples. This chloramphenicol extraction kit enables the separation of chloramphenicol from tissue and tissue water components. The extracted chloramphenicol can then be detected and quantified by enzyme-linked immunosorbent assay (ELISA) followed by spectrophotometric analysis. In the standard extraction, chloramphenicol is typically extracted using ethyl acetate. In this procedure, ethyl acetate containing the chloramphenicol is separated from tissue by centrifugation for 10 minutes followed by manual pipetting. An aliquot is then dried and reconstituted into 1 mL each of iso-octane:chloroform (2:3) solution and sample dilution buffer, of which 50 μL of sample dilution buffer is used for analysis by ELISA and spectrophotometry.