Triose Phosphate Isomerase Activity Colorimetric Assay Kit, ยี่ห้อ Sigma

PROPERTIES

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for detection of Triose Phosphate Isomerase in animal tissues (muscle, liver, heart, kidney, etc.), cell culture (adherent or suspension cells) and human serum or plasma.

Principle

This Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for determining triose phosphate isomerase activity in a variety of samples. In this kit, triose phosphate isomerase converts dihydroxyacetone phosphate into glyceraldehyde 3-phosphate, which reacts with the enzyme mix and developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, and high-throughput, and can detect triose phosphate isomerase activity as low as 40 mU/mL.