D-Gluconate (D-Gluconic Acid) Assay Kit (Colorimetric) ยี่ห้อ Sigma

PROPERTIES

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

D-Gluconic acid (C6H12O7) is a mild organic acid that occurs from the oxidation of glucose. It is naturally present in fruit, honey, and wine When used as a food additive, it helps regulate acidity. It is a strong chelating agent, chelating the anions of calcium, iron, aluminum, copper, and other heavy metals. D-Gluconate (C6H11O7) is the salt or ester of Gluconic Acid. Due to its low toxicity, it is widely used in pharmaceutical, food, and other industries.

Suitability

Suitable for the measurement of D-Gluconate (D-Gluconic Acid) in various samples such as tissues, plants, fruits and wine

Principle

The D-Gluconate (gluconic acid) Assay Kit is a simple and sensitive method for quantifying D-gluconate. In this assay, gluconate is utilized by glucokinase to form D-gluconate-6-phosphate and ADP producing a coupled enzyme reaction that generates a colorimetric signal (450 nm), proportional to the amount of D-gluconate present. The assay is sensitive to less than 2 μM of D-gluconate in a variety of samples.