Collagenase Activity Colorimetric Assay Kit, ยี่ห้อ Sigma

PROPERTIES

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren′s contracture, an affliction characterized by a thickening of connective tissue.

Suitability

Suitable for the measurement of collagenase activity in bacterial extract and for screening/studying/characterizing collagenase inhibitors in purified enzyme.

Principle

This Collagenase Activity Assay Kit provides a quick and easy way to determine activity of collagenase. The kit measures collagenase activity using a synthetic peptide (FALGPA) that mimics collagen′s structure. It is suitable for measuring activity of bacterial collagenases such as from Clostridium histolyticum. In addition, it can also be used to screen/characterize collagenase inhibitors. The limit of detection for this assay is 0.02 mU of collagenase.