NADP/NADPH Assay Kit, ยี่ห้อ Sigma

PROPERTIES

detection method

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and the redox state of cells or tissue.

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Features and Benefits

Sensitive and accurate - Detection limit of 0.01 μM and linearity up to 1 μM NADP+/NADPH in 96 well plate assay.

Convenient - The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.

High-throughput - Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.

Sensitive and accurate - Detection limit of 0.01 mM and linearity up to 1 mM NADP+ /NADPH in 96 well plate assay.

Convenient – The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.

High-throughput – Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.

Suitability

Suitable for measuring NADP+ /NADPH concentrations and ratio in cell or tissue extracts.

Principle

The NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lex = 530 nm/lem = 585 nm, is proportional to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ / NADPH and with minimal interference (<1%) by NAD+ /NADH. This assay is a convenient, direct method to measure NADP+ /NADPH concentrations and ratio in cell or tissue extracts.

Simple, direct and automation-ready procedures for measuring NADP+ /NADPH concentration are very desirable. This NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex = 530 nm/λem = 585 nm, is proportional to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+/ NADPH and with minimal interference (<1%) by NAD+ /NADH. This assay is a convenient, direct method to measure NADP+ /NADPH concentrations and ratio in cell or tissue extracts.