Nitric Oxide Synthase Inhibitor Screening Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

DESCRIPTION

General description

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO is the oxidation product of arginine by nitric oxide synthase (NOS) and is involved in host defense development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in research and drug discovery.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for inhibitor screening and evaluation of nitric oxide synthase inhibitors.

Principle

The Nitric Oxide Synthase Inhibitor Screening Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method.