Cell Cycle Analysis Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 assays

detection method

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Cell cycle is a ubiquitous, complex sequence of events leading to growth and proliferation of cells. Cell cycle progression is tightly regulated due to its involvement in development, DNA damage and repair, etc. Anomalies in cell cycle progression can lead to tissue hyperplasia and diseases such as cancer. Cell cycle can be subdivided into interphase (G0/G1, S and G2) and mitotic (M) phase (prophase, metaphase, anaphase and telophase).

Features and Benefits

The method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.

Suitability

Suitable for studying cell cycle in adherent or suspension cells

Principle

The kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry.