Fatty Acid Extraction Kit for Pet Food ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 40 extractions

greener alternative product characteristics

Waste Prevention

Learn more about the Principles of Green Chemistry.

storage temp.

room temp

DESCRIPTION

General description

Lipids in food serve as important energy substrates for growth and maintenance. Lipids in foods are composed primarily of triacylglycerides. Other minor components such as phospholipids, free fatty acids and cholesteryl esters may be present. The Fatty Acid Extraction Kit for Pet Food enables the extraction of these lipids in one step. The extracted lipids can then be transesterified and quantified using gas chromatography (GC) with flame-ionization detection (FID).

The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.

The Fatty Acid Extraction Kit for Pet Food shortens the extraction process by eliminating the need to centrifuge, pipette, and prepare solvents and standards. Once the sample is homogenized and dissolved in the Extraction Solvent containing the internal standard, it is vortexed and poured into the plunger syringe with filter, which preferentially elutes the chloroform layer containing total lipids. The user then squeezes the plunger to ensure that the lipids are eluted from the syringe filter. A portion of the total lipid extract containing the total fatty acids can then be transesterified for GC-FID analysis as described in the Procedure. Data comparing the standard Folch method to the Fatty Acid Extraction Kit for Pet Food extraction method are presented under the Results Section.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.

Suitability

This kit is suitable for the extraction of lipids from pet foods.

Other Notes

Experts have tested the Fatty Acid Extraction Kits. Watch the Video to learn more and to see how the technology works.