Lactose Assay Kit ยี่ห้อ Sigma

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General description

Lactose (C12H22O11) is a disaccharide comprised of galactose and glucose which form a β1-4 glycosidic linkage. Lactose is the major sugar in milk, making up around 2–8 % of milk (by weight).

Features and Benefits

·      Linear detection range of the kit is 17 to 2000 µM lactose for the colorimetric assay and 6 to 100 µM for the fluorometric assay.

Suitability

The kit is suitable for lactose activity determination in milk, food, beverages and other biological samples, as well as for studying the effects of drugs on lactose metabolism.

Principle

The Lactose Assay Kit uses a specific enzyme-coupled reaction in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the lactose concentration in the sample.