Isocitrate Dehydrogenase (IDH) Assay Kit ยี่ห้อ Sigma

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General description

Isocitrate dehydrogenase (IDH) is an enzyme which catalyzes the interconversion of isocitrate and α-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD⁺ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP⁺ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the IDH1 and IDH2 isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia.

Features and Benefits

·      Linear detection range 0.1 to 100 U/L for 30 minute reaction.

·      Compatible with high-throughput handling systems.

Suitability

The kit is suitable for IDH activity determination in biological samples (e.g. plasma, serum, tissue and culture media.)

Principle

The Isocitrate Dehydrogenase (IDH) Assay Kit is a non-radioactive, colorimetric IDH assay based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT, which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.