Oxaloacetate Assay Kit ยี่ห้อ Sigma

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General description

Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate.

Features and Benefits

·      Linear detection range: 7 to 400 µM for the colorimetric assay and 1 to 40 µM for the fluorometric assay.

Suitability

The kit is suitable for oxaloacetate determination in plasma, serum, tissue and culture media.

Principle

The Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the oxaloacetate concentration in the sample.