α-Ketoglutarate Quantitation Kit ยี่ห้อ Sigma

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General description

Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism. As a precursor of glutamate and glutamine, α-ketoglutarate is a central metabolic fuel for cells of the gastrointestinal tract as well. It can decrease protein catabolism and increase protein synthesis to enhance bone tissue formation in the skeletal muscles and can be used in clinical applications. Alpha-ketoglutarate is used for kidney disease; intestinal and stomach disorders, including bacterial infections; liver problems; cataracts; and recurring yeast infections. It is also used for improving the way kidney patients receiving hemodialysis treatments process protein.

Suitability

The α-Ketoglutarate Quantitation Kit offers a sensitive colorimetric assay for quantifying α-ketoglutarate in biological samples.

Principle

This assay utilizes an enzyme coupled reaction that releases hydrogen peroxide, which can be detected by the peroxidase substrate at an absorbance of 570 nm.