Beta-Lactamase Activity Assay Kit ยี่ห้อ Sigma

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General description

β-Lactamases are a large family of enzymes capable of hydrolyzing β-lactams. A β-Lactam ring is the common element in all beta-lactam antibiotics including penicillin derivatives, cephalosporins, monobactams, and carbapenems. Through hydrolysis, β-lactamase breaks the β-lactam ring open, deactivating the antibacterial properties. Bacteria from clinical and non-clinical settings are becoming increasingly resistant to β-lactam antibiotics by synthesizing β-lactamase. To overcome this resistance, β-lactam antibiotics are often given with β-lactamase inhibitors such as clavulanic acid. Therefore, detection of β-lactamase activity is of central importance to assess beta-lactam antibiotics as well as to prevent antibiotics resistance.

The Beta-Lactamase Activity Assay Kit offers a sensitive colorimetric assay for measuring β-lactamase activity in biological samples. The assay can be performed using an absorbance microplate reader by measuring the OD ratio at the wavelength of 490 nm to 380 nm.

Principle

The β-lactamase activity is detected using nitrocefin, which changes color from yellow to red upon hydrolysis by β-lactamase.

One unit of β-lactamase is the amount of enzyme required to hydrolyze 1.0 µmole of nitrocefin per minute at pH 7.0 at 25°C.