Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution ยี่ห้อ Sigma

PROPERTIES

Quality Level

200

form

DMSO solution

shipped in

dry ice

storage temp.

−20°C

DESCRIPTION

General description

The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Application

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

Features and Benefits

Broad specificity: Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Contains no metal chelators: Ensures compatibility with downstream applications

Convenient packaging: 1 mL in glass bottle

Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Components

Aprotinin

Bestatin

E-64

Leupeptin

Pepstatin A

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibtion of serine, cysteine, aspartic and aminopeptidases. Contains aprotinin, bestatin, leupeptin, E-64, and pepstatin A. Contains no metal chelators

Caution

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Quantity

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form

Solution in DMSO (D 2650, Hybri-Max).