Ponceau S, BioReagent, suitable for electrophoresis ยี่ห้อ Sigma

PROPERTIES

product line

BioReagent

Quality Level

200

form

powder

technique(s)

electrophoresis: suitable

solubility

H₂O: 1 mg/mL

ε (extinction coefficient)

≥330000 at 518-522 nm in deionized water at 0.015 g/L

application(s)

diagnostic assay manufacturing

hematology

histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

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DESCRIPTION

General description

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.[1]

The following are two common stain formulations:

0.1% Ponceau S (w/v) in 5% acetic acid

2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Application

Ponceau S has been used for membrane staining (Western blotting.[2][3][4]

SAFETY INFORMATION

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable