Phytagel BioReagent, suitable for plant cell culture, powder, ขนาด 250g, ยี่ห้อ Sigma-Merck

DESCRIPTION

• General description
• Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel^™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl₂ or MgSO₄) or higher concentrations of Phytagel.
• Application
• Phytagel^™ has been used:
• in the solidification of culture medium for root assays in Arabidopsis thaliana
• as a component of Solid Paul′s medium for Agrobacterium culture
• as a component of root induction medium in Ilex dumosa tissue culture
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• Packaging
• 100, 250, 500 g in poly bottle
• 1, 5 kg in poly drum
• Biochem/physiol Actions
• Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.
• Other Notes
• An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.
• Reconstitution
• To prevent clumping, add slowly to rapidly stirring medium prior to heating.
• Legal Information
• Phytagel is a trademark of Sigma-Aldrich Co. LLC