Pyruvate kinase M2 Active human ยี่ห้อ Sigma

PROPERTIES

biological source

human

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

58.9 kDa

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_002654

UniProt accession no.

P14618

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PKM2(5315)

DESCRIPTION

General description

Human Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_002654) full length with N-terminal His tag, MW = 58.9 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Pyruvate kinase M2 (PKM2) is an isoform of pyruvate kinase that is expressed in certain differentiated cells and in proliferatively active cells. PKM2 is often overexpressed in cancer cells.

Unit Definition

One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.