TNF-α (human) ยี่ห้อ Sigma

PROPERTIES

biological source

human

Quality Level

300

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

form

lyophilized

potency

≤0.10 ng/mL ED₅₀

mol wt

17.4 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg endotoxin

UniProt accession no.

P01375

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TNF(7124)

DESCRIPTION

General description

TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts.[1][2] It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2.[3] The gene is mapped to human chromosome 6p21.3.[4] Recombinant human TNF-α is a soluble 157 amino acid protein (17.4kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Application

TNF-α (tumor necrosis factor α) human has been used as a positive control for induction of proinflammatory genes in human intestinal and hepatic cell lines.[4]

Biochem/physiol Actions

TNF-α (tumor necrosis factor α) is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. However, it can be a tumor promoter as well as a tumor suppressor.[3][2] TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, apoptosis and diabetes.[5][6][7][8][9] It is also associated with mood and anxiety disorders.[10]

Sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

SAFETY INFORMATION

WGK

WGK 3