PDHK3, active, GST tagged human ยี่ห้อ Sigma

PROPERTIES

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

10.2-13.8 nmol/min·mg

mol wt

~69 kDa

NCBI accession no.

NM_005391

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PDK3(5165)

DESCRIPTION

Biochem/physiol Actions

PDHK3 or Pyruvate Dehydrogenase Kinase 3 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. HIF-1 induces PDHK3 expression leading to inhibition of mitochondrial respiration. Forced expression of PDHK3 in cancer cells results in increased lactic acid accumulation and drugs resistance, whereas knocking down PDHK3 inhibits hypoxia-induced cytoplasmic glycolysis and cell survival.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

SAFETY INFORMATION

Storage Class Code

10 - Combustible liquids

WGK

WGK 1