Total Protein Kit, Micro Lowry, Peterson’s Modification ยี่ห้อ Sigma
รหัสสินค้า : TP0300-1KT
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PROPERTIES
usage
sufficient for ~200 assays
Quality Level
DESCRIPTION
General description
This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.
Application
Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.[1][2][3]
Features and Benefits
· Complete kit for protein assays
· Most widely cited protein assay
· Less protein to protein variation than other assays
· Contains reagents that can remove interfering substances
Principle
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
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