TrueGel3D® Hydrogel Kits SLO-DEXTRAN, allows cell recovery, CD cell-degradable crosslinker ยี่ห้อ Sigma

General description

The gel is formed by stable thioether bonds between thiol groups on CD cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-Dextran does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step.

The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

TRUE7-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of mechanical stimuli on cell physiology and behavior. The SLO-DEXTRAN based hydrogels are used in microchannels and also in syringes for injection. These hydrogels are also employed to study cell migration of neutrophils, dendritic cells and lymphocytes.

Features and Benefits

·      Slow gelling (takes 2.5 minutes to 6 minutes, depends on gel stiffness)

·      Flexibility in modulating gel stiffness

·      Contains MMP-cleavable crosslinker

·      Allow cell recovery and post-culture analyses 

·      Cell inert hydrogel (if not customized with RGD peptide)

Components

TRUE7-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized CD cell-degradable crosslinker with reconstitution buffer and water.

Caution

Avoid prolonged exposure of CD cell-degradable crosslinker to air.