TrueGel3D® Polymer modified for fast gelling (FAST-DEXTRAN), allows cell recoveryยี่ห้อ Sigma

General description

Dextrose polymers are functionalized with thiol-reactive maleimide groups, which react rapidly with thiol groups of crosslinkers to form hydrogels in few seconds. The thiol-reactive maleimide groups are also compatible to conjugate polymers with bioactive factors. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels. The buffers (pH7.2 and pH 5.5) are used to control pH and osmotic conditions to optimize time for gelation.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

FAST-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

Features and Benefits

·      Contains dextrose polymer functionalized with maleimide groups for quick gelation

·      Degradable polymer

Components

TRUEDEXF contains FAST-DEXTRAN lyophilized with reconstitution buffer, TrueGel3D thioglycerol and water

Caution

Avoid frequent freeze-thaw cycles and store FAST-DEXTRAN in small portions