TrueGel3D® Polymer modified for slow gelling (SLO-DEXTRAN), allows cell recovery ยี่ห้อ Sigma

General description

Dextrose polymers are functionalized with thiol-reactive groups, which react at a slower rate with thiol groups of crosslinkers (CD/PEG crosslinkers) to form stable hydrogels. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of homogenous gel formation. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

SLO-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

Features and Benefits

·      Contains dextrose polymer functionalized with thiol reactive groups for slow gelation

·      Degradable polymer

Components

TRUEDEXS contains SLO-DEXTRAN solution in phosphate buffer, TrueGel3D thioglycerol and water

Caution

Avoid frequent freeze-thaw cycles and store SLO-DEXTRAN in small portions