Amicon® Ultra - 2 mL Filters, 24/pk ยี่ห้อ MERCK

PROPERTIES

material

polypropylene tube (for collection)

regenerated cellulose membrane

styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon^®

manufacturer/tradename

Amicon^® Ultra

parameter

2 mL sample volume

technique(s)

protein extraction: suitable

protein purification: suitable

L

119.7 mm

diam.

15.9 mm

filtration area

1 cm²

volume

2 mL

pore size

10 kDa NMWCO

shipped in

ambient

DESCRIPTION

General description

Amicon^® Ultra-2 centrifugal filter devices offer fast ultrafiltration. It has the potential to recover high concentration factors and easy concentrate from dilute and complex sample matrices. Amicon^® Ultra-2 devices are supplied non-sterile and are for single use only.

Application

·       Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel^® membrane.

·       Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.

·       Purification of macromolecular components found in tissue culture extracts or cell lysates.

·       Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.

·       Desalting, buffer exchange and protein dialysis.

·       Size exclusion and protein fractionation.

Amicon^® Ultra-2 Centrifugal Filter Unit has been used:

·       to reduce large volume biofluids prior to extracellular vesicle (EV) isolation or to concentrate EVs[1]

·       Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples

·       Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal before high-performance liquid chromatography (HPLC)

·       Desalting, buffer exchange[2], or diafiltration

Features and Benefits

·       Amicon^® Ultra 2ml 10K 24PK

·       Concentrates 2 mL down to 15-70 μl

·       98% sample recovery

·       Fast processing time - 10 to 30 minute spin times

·       Engineered dead stop

·       The reverse spin capability provides consistent recoveries

·       Ultracel^® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%

·       Concentrates 2 mL down to 15-70 μL

·       98% sample recovery

·       Fast processing time: 10 to 30-minute spin times

·       Engineered dead stop

·       Reverse spin capability provides consistent recoveries

Linkage

Replaces: 4205

Other Notes

The Amicon^® Ultra-2 device is supplied with two tubes. During operation, one tube is used to collect filtrate; the other to cap the device during concentration and subsequently to recover the concentrated sample. Amicon Ultra-2 Centrifugal filter devices are for research use only and not for use in diagnostic procedures.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany