Amicon® Ultra – 0.5 mL Filters, 24/pk ยี่ห้อ MERCK

PROPERTIES

material

polypropylene cap

polypropylene tube

regenerated cellulose membrane

styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon^®

manufacturer/tradename

Amicon^® Ultra

parameter

0.5 mL sample volume

technique(s)

protein extraction: suitable

protein purification: suitable

L

4.99 cm (1.96 in.)

diam.

10.8 mm

filtration area

1 cm²

volume

0.5 mL

pore size

10 kDa NMWCO

shipped in

ambient

DESCRIPTION

General description

Amicon^® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon^® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^® Ultra 0.5 mL filters deliver great performance and lower spin times.

Application

·       Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel^® membrane

·       Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms

·       Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC

·       Desalting, buffer exchange, and protein dialysis

Features and Benefits

·       Ultracel^® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%

·       Concentrates 500 μL down to 15 μL

·       Concentration factor 25x to 30x

·       High sample recovery (greater than 90% of dilute starting solution)

·       Fast processing time: 10 to 30 minutes

·       Reverse spin capability provides consistent recoveries

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany