Amicon® Ultra – 15 mL Filters, 24/pk ยี่ห้อ MERCK

PROPERTIES

material

polyethylene cap

polyethylene liner

polypropylene tube

regenerated cellulose membrane

regenerated cellulose support

styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon^®

manufacturer/tradename

Amicon^® Ultra

parameter

15 mL sample volume

technique(s)

protein extraction: suitable

protein purification: suitable

L

12.1 cm (4.807 in.)

diam.

2.97 cm

filtration area

7.6 cm²

volume

15 mL

pore size

10 kDa NMWCO

shipped in

ambient

DESCRIPTION

General description

Amicon^® Ultra 15 mL centrifugal devices with Ultracel^® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon^® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

·       Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel^® membrane.

·       Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.

·       Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.

·       Concentration of dilute or pre-purified proteins from column eluents.

·       Desalting, buffer exchange and protein dialysis.

Features and Benefits

·       Ultracel^® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.

·       Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes).

·       Heat-sealed membrane minimizes downstream extractables.

·       100% integrity tested for reliable performance.

·       Convenient sample monitoring with translucent housing and volume gradations.

·       Direct pipettor sample access eliminates processing step to recover concentrate.

·       High concentration factors of 80–100X.

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany