Aldolase activity colorimetric assay kit ยี่ห้อ Sigma

PROPERTIES

detection method

colorimetric

storage temp.

−20°C

Gene Information

human ... ALDOA(226) , ALDOB(229) , ALDOC(230)

mouse ... ALDOA(11674) , ALDOB(230163) , ALDOC(11676)

rat ... ALDOA(24189) , ALDOB(24190) , ALDOC(24191)

DESCRIPTION

General description

Aldolase (fructose-bisphosphate aldolase; EC 4.1.2.13) catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three isoforms of aldolase (A, B, and C) are differentially expressed during development. Aldolases have been recognized as positive regulators of the Wnt signaling pathway. Deficiency of aldolases is associated with myopathy and hemolytic anemia.

The Aldolase Activity Colorimetric Assay Kit is a simple and high throughput assay for measuring aldolase activity in serum, plasma, and a variety of tissues and cells. Aldolase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of aldolase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.2 at 37 °C.

Aldolase (fructose-bisphosphate aldolase; EC 4.1.2.13) catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three isoforms of aldolase (A, B, and C) are differentially expressed during development.[1][2] Aldolases have been recognized as positive regulators of the Wnt signaling pathway.[1] Deficiency of aldolases is associated with myopathy and hemolytic anemia[2][3]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of Aldolase activity in biological samples such as tissue, cells, serum, and plasma.

Principle

The Aldolase Activity Colorimetric Assay Kit is a simple and high throughput assay for measuring aldolase activity in serum, plasma, and a variety of tissues and cells. Aldolase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of aldolase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.2 at 37 °C.