Promega offers a variety of cloning enzymes including DNA and RNA ligases and polymerases. T7, T3, and SP6 RNA Polymerases are popular choices for in vitro transcription, as they are highly specific for their respective promoters, and produce high-quality transcripts that can later be used as templates for in vitro translation.

The cloning of a specific fragment of DNA into a vector such as a plasmid is one of the foundational steps that enables additional characterization of that sequence. Vectors such as the pGEM® series contain multiple cloning sites to excerpt fragments generated by digestion with specific restriction enzymes. Sequences created by PCR and related TA cloning methods can be easily ligated into the pGEM® T-vectors. For multiple downstream applications (i.e., protein expression) the unique Flexi® Cloning System can conveniently transfer fragments to multiple vectors.

We offer a wide variety of DNA and RNA ladders and markers for conventional gel electrophoresis applications. Many ladders are available ready-to-load, meaning loading dye is already added. Select ladders are shipped at ambient temperature, no longer requiring ice and foam coolers for transport. Broad Range Protein Molecular Weight markers are also available.