The cloning of a specific fragment of DNA into a vector such as a plasmid is one of the foundational steps that enables additional characterization of that sequence. Vectors such as the pGEM® series contain multiple cloning sites to excerpt fragments generated by digestion with specific restriction enzymes. Sequences created by PCR and related TA cloning methods can be easily ligated into the pGEM® T-vectors. For multiple downstream applications (i.e., protein expression) the unique Flexi® Cloning System can conveniently transfer fragments to multiple vectors.