ReliaPrep™ DNA Clean-Up and Concentration System

Concentrate DNA from large volumes to ≤15µl

• Column-based method for rapid concentration and cleanup of DNA
• High binding capacity to accommodate large samples
• Small elution volumes provide highly concentrated samples and remove inhibitors

Prepare DNA samples for downstream applications

The ReliaPrep™ DNA Clean-Up and Concentration System is designed to quickly concentrate and purify dilute DNA solutions, extract and purify DNA fragments of 100bp–10kb from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE) buffer, or to purify products directly from a PCR amplification. This membrane-based system can bind up to 60µg of DNA and concentrate as much as 300µl of dilute DNA, recovering isolated DNA fragments or PCR products in as little as 10 minutes, depending on the number of samples processed and the protocol used.

A single reagent stream is used for all three procedures, making the system both fast and easy. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, next-generation sequencing or in vitro transcription/translation.

The ReliaPrep™ DNA Clean-Up and Concentration System can be used with linear DNA fragments, supercoiled plasmid DNA or single-stranded linear or circular DNA.

ItemPart #SizeAvailable Separately

Column Wash Solution (CWE) A254C1 × 24ml

Buffer B (BWB) A288B1 × 58.8ml

Membrane Binding Solution A930B2 × 20mlView Product

Elution Tubes Z200C5 × 50/pk

ReliaPrep™ Minicolumns A207B5 × 50/pack

Nuclease-Free Water P119C1 × 25mlView Product

Collection Tubes A130B1 × 250/pk

แหล่งที่มา : https://worldwide.promega.com/products/nucleic-acid-extraction/clean-up-and-concentration/reliaprep-dnacleanup-and-concentration-system/?catNum=A2893