pF1A T7 Flexi(R) Vector, 20ug ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : C8441
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pF1 T7 Flexi® Vectors
Untagged Bacterial Expression Vectors
- Generate protein from T7 promoter in E. coli and cell-free expression systems
- Options for Kanamycin and Ampicillin resistance
- Starting vector for transferring ORFs into other Flexi® Vectors
F1A and pF1K T7 Flexi® Vectors are designed for untagged protein expression in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein.
About the Flexi® Vector System
The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.
ItemPart #Size
pF1A T7 Flexi® Vector
C844A1 × 20μg
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