pFN2A (GST) Flexi(R) Vector, 20ug ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : C8461
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pFN2 (GST) Flexi® Vectors
Protein Expression Vectors with an N-terminal GST Fusion Tag
- Express in E. coli or a cell-free expression system using the T7 promoter
- Use N-terminal glutathione-S-transferase tag to purify or bind fusion proteins
- Cleave GST from protein using TEV protease
The pFN2A and pFN2K (GST) Flexi® Vectors are used for inducible expression of GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The GST tag has been successfully used to boost tagged protein solubility during E. coli expression. Reusable gluthathione (GST-affinity) resins can be used with the pFN2 (GST) Flexi® Vectors for standard protein purification and prokaryotic expression applications. Express cloned GST-tagged ORFs in bacterial and cell-free systems.
About the Flexi® Vector System
The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.
ItemPart #Size
pFN2A (GST) Flexi® Vector
C846A1 × 20μg
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