Primer Extension System ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : E3030
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Primer Extension System—AMV Reverse Transcriptase
We are sorry, but this product is discontinued.
For more information, contact Technical Service.
Primer Extension System—AMV Reverse Transcriptase can be used to quantitate specific mRNA transcripts and map the start sites of transcription. An end-labeled oligonucleotide is hybridized to RNA and is used as a primer by reverse transcriptase in the presence of deoxynucleotides. The RNA is thus reverse transcribed into cDNA and is analyzed on a denaturing polyacrylamide gel. The length of the cDNA reflects the number of bases between the labeled nucleotide of the primer and the 5´-end of the RNA; the quantity of cDNA product is related to the amount of targeted RNA.
ItemPart #SizeAvailable Separately
1.2kb Kanamycin Positive Control RNA
C154A1 × 100ng
Loading Dye
E343B1 × 1ml
Primer Extension 2X Buffer
E349B1 × 500μl
Primer Extension Sodium Pyrophosphate
E350B1 × 75μl
ΦX174 DNA/HinfI Dephosphorylated Markers
E351A1 × 500ng
Primer Extension Control Primer
E355A1 × 50pmol
AMV Reverse Transcriptase
E357B1 × 50u
T4 Polynucleotide Kinase
M410A1 × 100uView Product
T4 Polynucleotide Kinase 10X Buffer
Q561A1 × 100μl
Nuclease-Free Water
P119A1 × 1,250μlView Product
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