pFN19A HaloTag® T7 SP6 Flexi®Vector, 20ug ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : G1891
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pFN19 and pFC20 HaloTag® T7 SP6 Flexi® Vectors
We are sorry, but these products are discontinued.
For more information, contact Technical Service.
The pFN19 and pFC20 HaloTag® T7 SP6 Flexi® Vectors feature inducible expression of HaloTag® fusion proteins in E. coli and T7 and SP6 cell-free systems. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein.
pFN19A/K HaloTag® T7 SP6 Flexi® Vectors (Cat.# G1891, G1841) are designed for protein expression with an N-terminal HaloTag® using the T7 or SP6 RNA polymerase promoter. pFC20A/K HaloTag® T7 SP6 Flexi® Vectors (Cat.# G1681, G1691) express protein with a C-terminal HaloTag® using the T7 and SP6 RNA polymerase promoter. Proteins expressed from these vectors can be purified using HaloTag® technology.
About the Flexi® Vector System
The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.
ItemPart #Size
pFN19A HaloTag® T7 SP6 Flexi® Vector
G189A1 × 20μg
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