HaloTag(R) Protein Purification System, Sample Pack ยี่ห้อ Promega (Stroge. Temp COMPONENTS AT DIFFERENT TEMPS)
รหัสสินค้า : G6270
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HaloTag® Protein Purification System
Enhanced Expression and Solubility of Recombinant Proteins with HaloTag® Ligands
- Covalent, efficient and specific capture of your protein of interest
- Overcome limitations (i.e., poor capture of proteins expressed at low levels, protein loss during washing) associated with affinity tags
- Sample size is available (choose Cat.# G6270)
Enhanced Expression and Solubility of Purified Protein with Novel Tag
HaloTag® Protein Purification System is designed to purify proteins fused to the HaloTag® novel protein tag that enhances the expression and solubility of recombinant proteins. HaloTag® Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin, thus overcoming the equilibrium-based limitations associated with affinity tags (i.e., poor capture of proteins expressed at low levels and protein loss during washing of the purification resin.
The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusion proteins as well as to monitor the efficiency of immobilization to HaloLink™ Resin by labeling with fluorescent HaloTag® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the HaloLink™ Resin Technical Manual #TM250, the HaloLink™ Protein Array Technical Manual #TM310 and the HaloCHIP™ System Technical Manual #TM075.
Use the HaloTag® Protein Purification System for:
- Purification of poorly expressed fusion proteins
- Improved yield, purity and specific activity of soluble, functional proteins compared to His-Tag, GST and MBP affinity tags
- Enhance target protein expression in prokaryotic, mammalian and cell-free systems
- Recover tag-free target protein using TEV Protease cleavage
- In-gel detection and quantification of protein expression levels with fluorescent HaloTag® Ligands
ItemPart #SizeAvailable Separately
TEV Protease for use with HisLink™ Resin
V109A1 × 200μl
HisLink™ Protein Purification Resin
V882D1 × 0.5mlView Product
HaloLink™ Resin
G19131 × 2.5ml
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