HaloTag(R) Protein Purification System ยี่ห้อ Promega (Stroge. Temp COMPONENTS AT DIFFERENT TEMPS)

รหัสสินค้า : G6280

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HaloTag® Protein Purification System

Enhanced Expression and Solubility of Recombinant Proteins with HaloTag® Ligands

  • Covalent, efficient and specific capture of your protein of interest
  • Overcome limitations (i.e., poor capture of proteins expressed at low levels, protein loss during washing) associated with affinity tags
  • Sample size is available (choose Cat.# G6270)

Enhanced Expression and Solubility of Purified Protein with Novel Tag

HaloTag® Protein Purification System is designed to purify proteins fused to the HaloTag® novel protein tag that enhances the expression and solubility of recombinant proteins. HaloTag® Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin, thus overcoming the equilibrium-based limitations associated with affinity tags (i.e., poor capture of proteins expressed at low levels and protein loss during washing of the purification resin.

The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusion proteins as well as to monitor the efficiency of immobilization to HaloLink™ Resin by labeling with fluorescent HaloTag® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the HaloLink™ Resin Technical Manual #TM250, the HaloLink™ Protein Array Technical Manual #TM310 and the HaloCHIP™ System Technical Manual #TM075.

Use the HaloTag® Protein Purification System for:

  • Purification of poorly expressed fusion proteins
  • Improved yield, purity and specific activity of soluble, functional proteins compared to His-Tag, GST and MBP affinity tags
  • Enhance target protein expression in prokaryotic, mammalian and cell-free systems
  • Recover tag-free target protein using TEV Protease cleavage
  • In-gel detection and quantification of protein expression levels with fluorescent HaloTag® Ligands


ItemPart #SizeAvailable Separately

TEV Protease for use with HisLink™ Resin

V109B1 × 1.6ml

HisLink™ Protein Purification Resin

V882C1 × 5mlView Product

HaloLink™ Resin

G19151 × 25ml


แหล่งที่มา : https://worldwide.promega.com/products/protein-purification/protein-purification-kits/halotag-protein-purification-system/?catNum=G6280

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