HaloTEV Protease

Cleave and Recover Target Protein in a Single Step

• Easy to covalently remove HaloTEV from your purified protein with HaloLink™ Resin
• Proteins can be purified tag-free in a single step as the HaloLink™ Resin will bind both HaloTag® protein tag and the HaloTEV protease
• Supplied at a concentration of 5u/μl

HaloTag®-Protease Fusion for Rapid Target Protein Recovery

HaloTEV Protease is a fusion between the HaloTag® protein and TEV protease, a highly specific proteolytic enzyme that cleaves at a specific TEV site, a specific seven-amino-acid sequence (ENLYFQ(G/S)). HaloTEV Protease enables covalent immobilization on HaloLink™ Resin and removal from a cleavage reaction in a single-step purification. The covalent capture of HaloTEV Protease improves purity of the final target protein and assures the improved recovery of the TEV protease.

It's easy to remove HaloTEV from your purified protein with HaloLink™ Resin.

References

1. Ryu, M.-J. et al. (2012) Oncogenic KRas expression in postmitotic neurons leads to S100A8-S100A9 protein overexpression and gliosis. J. Biol. Chem. 287, 22948–58.
2. Ohana, R.F. et al. (2011) HaloTag-based purification of functional human kinases from mammalian cells. Protein Expr. Purif. 76, 154–64.

ItemPart #SizeConcentration

HaloTEV Protease

G660A1 × 200μl5u/μl

แหล่งที่มา : https://worldwide.promega.com/products/protein-purification/protein-purification-kits/halotev-protease/?catNum=G6601