HaloTag® Mammalian Protein Purification System

Recover Highly Pure Recombinant Protein from Mammalian Cell Cultures, Even at Low Expression Levels

• HaloLink™ Resin covalently binds >7mg/ml of HaloTag® fusion protein (10X more capacity than FLAG®)
• Easy to scale up or down to obtain milligram + quantities of protein
• Choose from purification, purification + detection or sample purification + detection kits

Streamline Your Protein Purification Process

The HaloTag® Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag® fusion proteins from mammalian cell culture lysates. HaloTag® fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795 or G6799) also includes HaloTag® TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag® fusion facilitates rapid optimization of expression and purification conditions.

ItemPart #SizeConcentrationAvailable Separately

HaloLink™ Resin

G19131 × 2.5ml

Protease Inhibitor Cocktail, 50X

G652A2 × 1mlView Product

HaloTEV Protease

G660A1 × 200μl5u/μlView Product

Spin Column

V995A1 × 25 each

แหล่งที่มา : https://worldwide.promega.com/products/protein-purification/protein-purification-kits/halotag-mammalian-protein-purification-system/?catNum=G6790