A bioluminescent reporter assay consists of both a luciferase reporter enzyme and a detection reagent that provides the enzyme substrate. When the reporter enzyme and detection reagent are combined, the light emitted is proportional to reporter gene expression levels and is detected using a luminometer. We offer bioluminescent reagents for detecting NanoLuc®, firefly, and Renilla luciferases, with multiple detection reagent options for each enzyme to create a reporter assay optimized for specific experimental goals. The detection reagents have varying signal brightness and stability, include both lytic or live-cell options, and can provide either single- or dual-reporter detection.

Firefly luciferase can be paired with either NanoLuc® luciferase and assayed using the Nano-Glo® Dual-Luciferase® (NanoDLR™) Reporter Assay System, or paired with Renilla luciferase and detected using the Dual-Luciferase® Reporter Assay System. Using two genetic reporters creates a reporter assay that includes a normalization control or a second experimental reporter for more information per assay.